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Image Search Results
Journal: Nature
Article Title: EccDNAs are apoptotic products with high innate immunostimulatory activity
doi: 10.1038/s41586-021-04009-w
Figure Lengend Snippet: a , Representative gel image showing selective binding of circular DNA to magnetic silica beads in solution A. The assay was tested with 200× (mass) linear DNA (lane 4 and 6) and 1X (mass) DNA circles (lane 5 and 6). Lanes 1–3 indicate the input DNAs. Lane 1, 0.5% of input linear DNA for lane 4 was loaded; lane 2, equal amount of input circular DNAs for lane 5 were loaded; lane 3, 0.5% of input linear DNA and equal amount of input circular DNAs for lane 6 were loaded. Lanes 4–6 were DNAs recovered from Solution A. lane 4, 200× (mass) linear DNA alone undergone purification by solution A; lane 5, 1× (mass) DNA circles undergone purification by solution A; lane 6 mixture of 200× (mass) linear DNA and 1× (mass) DNA circles undergone purification by solution A. Note, only circular DNAs were recovered. The recovery rate is very high, particularly for the smaller circular DNAs. b , High sensitivity of DNA detection using vertical agarose gel electrophoresis and SYBR Gold staining. 0.2 μl commercial DNA ladder (total of 10 ng) was undergone 5× series dilutions and fractionated by 1% vertical agarose gel electrophoresis, stained by SYBR Gold, DNA in lane-4 were 125× diluted of that in lane-1, and could still be visualized. The detection limit is estimated as 5–10 pg/band. Results in a–b are representative of three independent experiments.
Article Snippet: All DNA, except the PCR genotyping, were resolved with vertical agarose gel electrophoresis and visualized by
Techniques: Binding Assay, Purification, Agarose Gel Electrophoresis, Staining